期刊
CHEMISTRYOPEN
卷 6, 期 6, 页码 739-750出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/open.201700143
关键词
collisionally activated dissociation; electrospray ionization; guanidinium; mass spectrometry; RNA
资金
- Austrian Science Fund (FWF) [P27347, P30087]
- Austrian Science Fund (FWF) [P27347] Funding Source: Austrian Science Fund (FWF)
Interactions of ribonucleic acid (RNA) with guanidine and guanidine derivatives are important features in RNA-protein and RNA-drug binding. Here we have investigated noncovalently bound complexes of an 8-nucleotide RNA and six different ligands, all of which have a guanidinium moiety, by using electrospray ionization (ESI) and collisionally activated dissociation (CAD) mass spectrometry (MS). The order of complex stability correlated almost linearly with the number of ligand atoms that can potentially be involved in hydrogen-bond or salt-bridge interactions with the RNA, but not with the proton affinity of the ligands. However, ligand dissociation of the complex ions in CAD was generally accompanied by proton transfer from ligand to RNA, which indicated conversion of salt-bridge into hydrogen-bond interactions. The relative stabilities and dissociation pathways of [RNA+mL-nH](n-) complexes with different stoichiometries (m=1-5) and net charge (n= 2-5) revealed both specific and unspecific ligand binding to the RNA.
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