期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1862, 期 3, 页码 324-336出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2016.12.004
关键词
Beta-oxidation; Fatty acid metabolism; Lipids; Triacylglycerol synthesis; Fatty acid oxidation; Insect physiology
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [308119/2013-4]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) [201.591/2014]
- Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular (INCT-EM) [573959/2008-0]
- U.S. National Institutes of Health (NIH) [DK56598, K08DK090141]
Glycerol-3-phosphate acyltransferases (GPAT) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (TAG). Four mammalian GPAT isoforms have been identified: the mitochondria-associated GPAT1 and 2, and the endoplasmic reticulum (ER)-associated GPAT3 and 4. In the insect Rhodnius prolixus, a vector of Chagas' disease, we previously predicted a mitochondrial-like isoform (RhoprGPAT1) from genomic data. In the current study, we clone the RhoprGPAT1 coding sequence and identify an ER-associated GPAT (RhoprGPAT4) as the second isoform in the insect. RhoprGPAT1 contributes 15% of the total GPAT activity in anterior midgut, 50% in posterior midgut and fat body, and 70% in the ovary. The RhoprGpat1 gene is the predominant transcript in the midgut and fat body. To evaluate the physiological relevance of RhoprGPAT1, we generate RhoprGPAT1-deficient insects. The knockdown of RhoprGpat1 results in 50% and 65% decrease in TAG content in the posterior midgut and fat body, respectively. RhoprGpat1-deficient insects also exhibits impaired lipid droplet expansion and a 2-fold increase in fatty acid beta-oxidation rates in the fat body. We propose that the RhoprGPAT1 mitochondrial-like isoform is required to channel fatty acyl chains towards TAG synthesis and away from beta-oxidation. Such a process is crucial for the insect lipid homeostasis. (C) 2016 Elsevier B.V. All rights reserved.
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