期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 8, 页码 6796-6803出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b14176
关键词
alkaline phosphatase; near-infrared; fluorescent probe; bioimaging; mice
资金
- Research Grants Council of Hong Kong [11302415, 21300714]
- National Natural Science Foundation of China [21202137, 21572190, 21390411]
Alkaline phosphatases are a group of enzymes that play important roles in regulating diverse cellular functions and disease pathogenesis. Hence, developing fluorescent probes for in vivo detection of alkaline phosphatase activity is highly desirable for studying the dynamic phosphorylation in living organisms. Here, we developed the very first reaction based near-infrared (NIR) probe (DHXP) for sensitive detection of alkaline phosphatase activity both in vitro and in vivo. Our studies demonstrated that the probe displayed an up to 66-fold fluorescence increment upon incubation with alkaline phosphatases, and the detection limit of our probe was determined to be 0.07 U/L, which is lower than that of most of alkaline phosphatase probes reported in literature. Furthermore, we demonstrated that the probe can be applied to detecting alkaline phosphatase activity in cells and mice. In addition, our probe possesses excellent biocompatibility and rapid cell-internalization ability. In light of these prominent properties, we envision that DHXP will add useful tools for investigating alkaline phosphatase activity in biomedical research.
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