期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1858, 期 2, 页码 175-181出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2016.11.010
关键词
Complex I assembly; N7BML; NDUFAF2; Iron sulfur cluster; NADH:ubiquinone oxidoreductase ROS
资金
- German Research Foundation [ZI 552/4-1, SFB815]
- Excellence Initiative of the German Federal and State Governments [EXC 115]
Mitochondria! complex I is an intricate 1 MDa membrane protein complex with a central role in aerobic energy metabolism. The minimal form of complex I consists of fourteen central subunits that are conserved from bacteria to man. In addition, eukaryotic complex I comprises some 30 accessory subunits of largely unknown function. The gene for the accessory NDUFS4 subunit of human complex I is a hot spot for fatal pathogenic mutations in humans. We have deleted the gene for the orthologous NUYM subunit in the aerobic yeast Yarrowia lipolytica, an established model system to study eukaryotic complex I and complex I linked diseases. We observed assembly of complex I which lacked only subunit NUYM and retained weak interaction with assembly factor N7BML (human NDUFAF2). Absence of NUYM caused distortion of iron sulfur clusters of the electron input domain leading to decreased complex I activity and increased release of reactive oxygen species. We conclude that NUYM has an important stabilizing function for the electron input module of complex I and is essential for proper complex I function. (C) 2016 Elsevier B.V. All rights reserved.
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