期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷 204, 期 -, 页码 65-75出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2016.11.008
关键词
Acid-base balance; Air exposure; Ammonia sensing; Behaviour; Carcinus maenas
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant [RGPIN473-12]
- International Development Research Centre (IDRC, Ottawa, Canada) [104519-003]
The physiological effects of high environmental ammonia (HEA) exposure have been well documented in many aquatic species. In particular, it has recently been demonstrated that exposure to ammonia in fish leads to a similar hyperventilatory response as observed during exposure to hypoxia. In littoral crabs, such as the green crab (Carcinus maenas), exposure to severe hypoxia triggers an emersion response whereby crabs escape hypoxia to breathe air. We hypothesized that exposure to HEA in green crabs would lead to a similar behavioural response which is specific to ammonia. Using an experimental arena containing a rock bed onto which crabs could emerse, we established that exposure to HEA (4 mmol/I NH4HCO3) for 15 min triggers emersion in crabs. In experiments utilizing NaHCO3 controls and NH4HCO3 injections, we further determined that emersion was triggered specifically by external ammonia and was independent of secondary acid-base or respiratory disturbances caused by HEA. We then hypothesized that emersion from HEA provides a physiological benefit, similar to emersion from hypoxia. Exposure to 15 min of HEA without emersion (no rock bed present) caused significant increases in arterial haemolymph total ammonia (T-amm), pH, and [HCO3-]. When emersion was allowed, arterial haemolymph T-amm and [HCO3-]increased, but no alkalosis developed. Moreover, emersion decreased haemolymph partial pressure of NH3 relative to crabs which could not emerse. Overall, we demonstrate a novel behavioural response to HEA exposure in crabs which we propose may share similar mechanistic pathways with the emersion response triggered by hypoxia. (C) 2016 Elsevier Inc. All rights reserved.
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