期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 765, 期 -, 页码 179-187出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2015.08.031
关键词
Interval exercise training; miR-21; Tamoxifen; Breast cancer
资金
- Tehran University of Medical Sciences [16943]
- Golestan University of Medical Sciences [910830171]
Exercise training has an anti-tumor effect and can reduce tumor growth; however, the exact underlying mechanisms of its protective effects are still obscure. MicroRNA (miR)-21 is a predictor in cancer survival, and has a potential use as an indicator of therapeutic outcome in breast malignancies. Forty-eight female BALB/c mice were equally divided into six groups to investigate the effects of interval exercise training with tamoxifen on miR-21 expression and its possible assumed mechanisms in an estrogen receptor-positive breast cancer model. ELISA, immunohistochemistry, western blot, qRT-PCR assays were performed at the end of the study. Tumor size was significantly declined in exercise training and tamoxifen groups compared to tumor group (P < 0.05). Expression of miR-21 was significantly down-regulated in trained and tamoxifen treated mice in comparison with tumor group (P < 0.05). Exercise training was as effective as tamoxifen treatment in decreasing serum estradiol and ER-alpha expression (P < 0.05). Exercise training and tamoxifen reduced tumor IL-6 levels, NF-kB and STAT3 expressions, and up-regulated TPM1 and PDCD4 expressions (P < 0.05). Both exercise and tamoxifen had synergistic effects in reducing miR-21 and Bcl-2, and up-regulating PDCD4 expression. Results showed that interval exercise training may reduce mammary tumor burden in mice through possible underlying pathway of miR-21. (C) 2015 Elsevier B.V. All rights reserved.
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