期刊
CELL STEM CELL
卷 20, 期 3, 页码 385-+出版社
CELL PRESS
DOI: 10.1016/j.stem.2016.11.017
关键词
-
资金
- Simons Foundation postdoctoral fellowship
- International Rett Syndrome Foundation postdoctoral fellowship
- NARSAD Young Investigator Grant from the Brain and Behavior Research Foundation
- European Leukodystrophy Association
- Jerome and Florence Brill Graduate Student Fellowship
- Simons Foundation [SFARI 204106, SFLIFE 286977]
- NIH [HD 045022, R01-MH104610, R01-NS088538, AI100190]
- ELA Foundation
- Emerald Foundation
- Biogen
- Simons Center for the Social Brain at MIT
- MRC [MC_UP_1201/9] Funding Source: UKRI
- Medical Research Council [MC_UP_1201/9] Funding Source: researchfish
An expansion of the cerebral neocortex is thought to be the foundation for the unique intellectual abilities of humans. It has been suggested that an increase in the proliferative potential of neural progenitors (NPs) underlies the expansion of the cortex and its convoluted appearance. Here we show that increasing NP proliferation induces expansion and folding in an in vitro model of human corticogenesis. Deletion of PTEN stimulates proliferation and generates significantly larger and substantially folded cerebral organoids. This genetic modification allows sustained cell cycle re-entry, expansion of the progenitor population, and delayed neuronal differentiation, all key features of the developing human cortex. In contrast, Pten deletion in mouse organoids does not lead to folding. Finally, we utilized the expanded cerebral organoids to show that infection with Zika virus impairs cortical growth and folding. Our study provides new insights into the mechanisms regulating the structure and organization of the human cortex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据