期刊
ANALYTICA CHIMICA ACTA
卷 953, 期 -, 页码 87-94出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.11.052
关键词
Brain extracellular fluid; Diffusible; Inductively coupled plasma mass spectrometry; Iron speciation; Packed minicolumn
资金
- Ministry of Science and Technology (R.O.C.) [MOST 104-2113-M-019-003-MY2, MOST 105-2627-M-007-010]
Exploration of brain extracellular non-protein-bound/diffusible iron species remains a critically important issue in investigations of free radical biology and neurodegenerative diseases. In this study, a facile sample pretreatment scheme, involving poly(vinyl chloride)-metal ion interactions as a selective extraction procedure, was optimized in conjunction with microdialysis (MD) sampling and inductively coupled plasma mass spectrometry (ICP-MS) in cool-plasma mode for in vivo online monitoring of rat brain extracellular Fe(II) and Fe(III) species. Optimization of the system provided detection limits in the range 0.9-6.9 mu g Fe L-1, based on a 12-mu L microdialysate, for the tested iron species; relative standard deviations of the signal intensities during 7.8 h of continuous measurement were less than 9.4%-sufficient to determine the basal concentrations of rat brain extracellular Fe(II) and Fe(III) species and to describe their dynamic actions. The method's applicability was verified through (i) spike analyses of offline-collected rat brain microdialysates, (ii) determination of the basal Fe(II) and Fe(III) concentrations of living rat brain extracellular fluids, and (iii) monitoring of the dynamic changes in the Fe(II) and Fe(III) concentrations in response to perfusion of a high -K+ medium. This proposed sample pretreatment scheme, based on polymer -metal ion interactions and hyphenation to an MD sampling device and an ICP-MS system, appears to have great practicality for the online monitoring of rat brain extracellular diffusible iron species. (C) 2016 Elsevier B.V. All rights reserved.
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