期刊
BIOMATERIALS
卷 118, 期 -, 页码 1-15出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.11.054
关键词
Thymus; Thymic epithelial cells; Decellularized thymus; Thymus organoids; Thymus regeneration; Thymus extracellular matrix; 3D culture system
资金
- Victoria-California Stem Cell Alliance Grant Program
- ARC [DP160101591]
Although the thymus is a primary lymphoid organ, its function is compromised by an age-induced loss of resident epithelial cells, which results in reduced na ve T cell output. This has important implications for immune recovery in aged and elderly patients following damage from cytoablative therapies. As thymic architecture plays a crucial role in na ve T cell development, a tissue specific scaffold that provides essential supporting matrix may assist in stem cell-based thymus regeneration to recreate complex organoids. Here we investigate thymus decellularization approaches that preserve major extracellular matrix components and support thymic epithelial cells for the generation of a functional thymic microenvironment with improved T cell output. We also established an in vitro, serum-free culture system that both maintains a progenitor thymic epithelial cell pool and drives their differentiation in the presence of decellularized thymic matrix. This approach enables further dissection of key cellular and niche components involved in thymic epithelial stem cell maintenance and T cell production. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据