期刊
BIOTECHNOLOGY JOURNAL
卷 13, 期 3, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700542
关键词
biocatalysis; immobilization; Pictet-Spengler-reaction; norcoclaurine synthase; flow chemistry
资金
- Austrian Science Fund (FWF) [W9-01]
- BBSRC [BB/G014426/1]
- Austrian Science Fund (FWF) [W 901] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/G014426/1, BB/N01877X/1] Funding Source: researchfish
- BBSRC [BB/N01877X/1, BB/G014426/1] Funding Source: UKRI
Norcoclaurine synthases (NCS), catalyzing a Pictet-Spengler reaction in plants as one of the first enzymes in the biosynthetic benzylisoquinoline pathway, are investigated for biocatalytic transformations. The library of NCS available is extended by two novel NCSs from Argemone mexicana (AmNCS1, AmNCS2) and one new NCS from Corydalis saxicola (CsNCS); furthermore, it is shown that the NCS from Papaver bracteatum (PbNCS) is a highly productive catalyst leading to the isoquinoline product with up to >99% e.e. Under certain conditions lyophilized whole Escherichia coli cells containing the various overexpressed NCS turned out to be suitable catalysts. The reaction using dopamine as substrate bears several challenges such as the spontaneous non-stereoselective background reaction and side reactions. The PbNCS enzyme is successfully immobilized on various carriers whereby EziG3 proved to be the best suited for biotransformations. Dopamine showed limited stability in solution resulting in the coating of the catalyst over time, which could be solved by the addition of ascorbic acid (e.g., 1mgml(-1)) as antioxidant.
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