期刊
BIOTECHNOLOGY JOURNAL
卷 13, 期 1, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700417
关键词
3D cell culture; high-throughput screening; liquid overlay technique; scaffold-free techniques; tumor spheroids
资金
- FEDER funds through the POCI - COMPETE - Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation [POCI-01-0145-FEDER-007491]
- National Funds by FCT - Foundation for Science and Technology [UID/Multi/00709/2013]
- [SFRH/BD/109482/2015]
- [SFRH/BD/103506/2014]
- [SFRH/BD/103507/2014]
Scalable and reproducible production of 3D cellular spheroids is highly demanded, by pharmaceutical companies, for drug screening purposes during the pre-clinical evaluation phase. These 3D cellular constructs, unlike the monolayer culture of cells, can mimic different features of human tissues, including cellular organization, cell-cell and cell-extracellular matrix (ECM) interactions. Up to now, different techniques (scaffold-based and -free) have been used for spheroids formation, being the Liquid Overlay Technique (LOT) one of the most explored methodologies, due to its low cost and easy handling. Additionally, during the last few decades, this technique has been widely investigated in order to enhance its potential for being applied in high-throughput analysis. Herein, an overview of the LOT advances, practical approaches, and troubleshooting is provided for those researchers that intend to produce spheroids using LOT, for drug screening purposes. Moreover, the advantages of the LOT over the other scaffold-free techniques used for the spheroids formation are also addressed. Highlights center dot 2D cell culture drawbacks are summarized; center dot spheroids mimic the features of human tissues; center dot scaffold-based and scaffold-free technologies for spheroids production are discussed; center dot advantages of LOT over other scaffold-free techniques are highlighted; center dot LOT advances, practical approaches and troubleshooting are underlined.
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