期刊
BIOMED RESEARCH INTERNATIONAL
卷 2017, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2017/4150705
关键词
-
资金
- Jiangsu Province Key Scientific and Technological Project [BE2016669]
- National Natural Science Foundation of China [81120108003, 81330007]
- Suzhou Science and Technology Project [SS201665]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Province's Key Discipline/Laboratory of Medicine [XK201118]
Mesenchymal stem cells (MSCs) repair infarcted heart through paracrine mechanism. We sought to compare the effectiveness of MSCs and MSC-derived exosomes (MSC-Exo) in repairing infarcted hearts and to identify how MSC-Exo mediated cardiac repair is regulated. In a rat myocardial infarction model, we found that MSC-Exo inhibited cardiac fibrosis, inflammation, and improved cardiac function. The beneficial effects of MSC-Exo were significantly superior compared to that of MSCs. To explore the potential mechanisms underlying MSC-Exo's effects, we performed several in vitro experiments and miRNA-sequence analysis. MSC-Exo stimulated cardiomyocyte H9C2 cell proliferation, inhibited apoptosis induced by H2O2, and inhibited TGF-beta induced transformation of fibroblast cell into myofibroblast. Importantly, novel miRNA sequencing results indicated that MSC-Exo and MSCs have similar miRNA expression profile, which could be one of the reasons that MSC-Exo can replace MSCs for cardiac repair. In addition, the expression of several miRNAs from MSC-Exo was significantly different from that of MSCs, which may explain why MSC-Exo has better therapeutic effect than MSCs. In conclusion, this study demonstrates that MSC-Exo could be used alone to promote cardiac repair and are superior to MSCs in repairing injured myocardium.
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