期刊
ADVANCED FUNCTIONAL MATERIALS
卷 27, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201604260
关键词
cell sheet harvesting; conducting polymers; near IR light diffraction; photothermal PEDOT; thermal patterns
类别
资金
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI15C0942]
- National Research Foundation (NRF) - Korean government (Ministry of Science, ICT & Future Planning, MSIP) through the Active Polymer Center for Pattern Integration (APCPI) [2007-0056091]
- Korea Health Promotion Institute [HI15C0942020017] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Near infrared (NIR) photothermal pattern on conductive polymer film enables unique approaches to harvest large-area cell sheets with various patterns without the use of patterned culture dish. The NIR photothermal pattern is generated from a patterned optical lens (POL), which creates a dynamic near IR light pattern and the corresponding photothermal pattern (PTP) on the polymer film. The POL is prepared from transparent polydimethylsiloxane designed to generate various light patterns. The PTPs allow a noninvasive harvest of cultured cells as an intact living cell sheet with a high harvesting efficiency ((cell) > 100). Various PTPs are generated by the diffraction of NIR light through POLs having different micropatterns, which afford cell sheets with a desired pattern without changing the original cell morphology at cultured state. Furthermore, a large-area living cell sheet is obtained with a detached area larger than 19 cm(2), which is the largest living cell sheet up to date. Further optical engineering of the harvesting system allows multiple productions of cell sheets with one dose of light. It is possible to harvest cell sheets not only from human fibroblast cells but also from human adipose-derived stem cells, indicating that the method can be applied to engineer various cells.
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