期刊
CHEMICAL ENGINEERING SCIENCE
卷 161, 期 -, 页码 48-56出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ces.2016.12.014
关键词
Enzymatic saccharification; Ionic liquids; Lignocellulosic biomass; Non-productive enzyme adsorption; Pretreatment
资金
- Natural Science Foundation of Guangdong Province [2014A030313263]
To provide deep insights into cholinium ionic liquid (IL) pretreatment effect on the subsequent enzymatic hydrolysis, residual lignins (RLs) present in rice straw pretreated by cholinium glycinate aCh][Gly]), acetate ([Ch][OAc]), and glycolate ([ Ch][Glc]) were isolated and characterized in this work. The effects of RLs on the enzymatic hydrolysis of cellulose and cellobiose, and their non-productive adsorption to beta-glucosidase and cellulase were explored. Of the three RLs examined, RL isolated from [Ch][Gly1pretreated straw (Gly-RL) had the highest carboxylic acid content (1.27 mmol/g) and the lowest phenolic hydroxyl content (0.72 mmol/g), resulting in the weakest non-productive enzyme adsorption. It might account well for the highest enzymatic digestibility of cellulose in the presence of Gly-RL. However, the strongest enzyme adsorption was found in the case of Glc-RL with the lowest carboxylic acid content (0.14 mmol/g) and the highest phenolic hydroxyl content (1.19 mmol/g). The enzymatic hydrolysis of cellulose was significantly affected by RLs. The negative effect of RLs on the cellulose hydrolysis mainly stemmed from their inhibition to cellulase-mediated reactions, instead of inhibition to beta-glucosidase-catalyzed cellobiose hydrolysis. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据