期刊
BIOMED RESEARCH INTERNATIONAL
卷 2017, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2017/5343010
关键词
-
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [25288075]
- Grants-in-Aid for Scientific Research [17K13221] Funding Source: KAKEN
It is well known that oxidative stresses induce the production of amyloid beta(A beta) in the brain, lens, and retina, leading to age-related diseases. In the present study, we investigated the effects of ferulic acid on the A beta levels in H2O2 - stimulated human lens epithelial (HLE) SRA01/04 cells. Three types of A beta peptides (A beta(1- 40), A beta(1- 42), andA beta(1- 43)) were measured by ELISA, and the levels of mRNA for the expressed proteins related to A beta production (APP, BACE1, and PS proteins) and degradation (ADAM10, NEP, and ECE1 proteins) were determined by quantitative real-time RT-PCR. H2O2 stimulation augmented gene expression of the proteins related to A beta production, resulting in the production of three types of A beta peptides. Treatment with 0.1 mu M ferulic acid attenuated the augmentations of gene expression and production of the proteins related to the secretion of three types of A beta peptides in the H2O2 - stimulated HLE cells. These results provided evidence of antioxidative functions of ferulic acid for lens epithelial cells.
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