期刊
BIOSENSORS & BIOELECTRONICS
卷 89, 期 -, 页码 964-969出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.09.078
关键词
Vascular endothelial growth factor (VEGF); Aptamer; Fluorescence biosensor; Isothermal amplification
类别
资金
- Chongqing Yuzhong District Science and Technology Project [20140108]
- Chongqing Medical Scientific Research Projects [20142167]
- Natural Science Foudation of Inner Mongolia [2015MS0850]
Vascular endothelial growth factor (VEGF) is an important biomarker in cancer angiogenesis. Here, we develop a aptasensor method for VEGF detection based on DNA assembly structure switching and isothermal amplification. The design employs a DNA assembly made of a isothermal amplification template, a aptamer, a primer and a protector chain. The DNA assembly is unable to undergo isothermal amplification in the absence of target. The presence of the target, however, triggers a structure switching event that causes hybridization of primer with template to facilitate isothermal amplification. Whereafter, toehold-mediated DNA strand displacement reaction between the generated (single-stranded DNA) ssDNA and fluorescent/quencher labeled probe are performed. Then, the increase in fluorescence provides an analytical signal. This strategy opens up a sensitive, selective and simple sensing platform for detection of VEGF. The system was also implemented to analyze the VEGF in human serum samples with satisfactory results.
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