期刊
BIOSENSORS & BIOELECTRONICS
卷 89, 期 -, 页码 959-963出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.09.080
关键词
Alzheimer's disease; alpha-1 antitrypsin; Sandwich assays; Antibody functionalized silver nanoparticles; Electrochemical biosensors
类别
资金
- National Research Foundation (NRF) of Korea - Ministry of Science, ICT, and Future Planning [NRF-2015R1A2A1A15052198]
A novel sandwich-type biosensor was developed for the electrochemical detection of alpha-1 antitrypsin (AAT, a recognized biomarker for Alzheimer's disease). The biosensor was composed of 3, 4, 9, 10-perylene tetracarboxylic acid/carbon nanotubes (PTCA-CNTs) as a sensing platform and alkaline phosphatase-labeled AAT antibody functionalized silver nanoparticles (ALP-AAT Ab-Ag NPs) as a signal enhancer. CNTs offer high surface area and good electrical conductivity. Importantly, Ag NPs could increase the amount of ALP on the sensing surface and the ALP could dephosphorylate 4-amino phenyl phosphate (APP) enzymatically to produce electroactive species 4-aminophenol (AP). For detecting AAT based on the sandwich-type biosensor, the results show that the peak current value of AP using ALP-AAT Ab-Ag NPs as signal enhancer is much higher than that by using ALP-AAT Ab bioconjugate (without Ag NPs), the biosensor exhibited desirable performance for AAT determination with a wide linearity in the range from 0.05 to 20.0 pM and a low detection limit of 0.01 pM. Finally, the developed sensor was successfully applied to the analysis of AAT concentration in serum samples.
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