期刊
BIOSENSORS & BIOELECTRONICS
卷 89, 期 -, 页码 989-997出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.10.051
关键词
Hollow molybdenum disulfide microcubes; Duplex-specific nuclease; MicroRNAs; Enzyme signal amplification; Electrochemical biosensor
类别
资金
- National Natural Science Foundation of China [U1304214, 21475115]
- Program for University Innovative Research Team of Henan [15IRTSTHN001]
- Henan Provincial Science and Technology Innovation team [C20150026]
- University Students Sustentation Fund of Xinyang Normal University [2016-DXS-23]
- Science and Technology Major Project of Henan Province [141100310600]
- Nanhu Scholars Program of XYNU
An ultrasensitive electrochemical biosensor for detecting microRNAs is fabricated based on hollow molybdenum disulfide (MoS2) microcubes. Duplex-specific nuclease, enzyme and electrochemical chemical chemical redox cycling are used for signal amplification. Hollow MoS2 microcubes constructed by ultrathin nanosheets are synthesized by a facile template-assisted strategy and used as supporting substrate. For biosensor assembling, biotinylated s5DNA capture probes are first immobilized on Au nanoparticles (AuNPs)/MoS2 modified electrode in order to combine with streptavidin-conjugated alkaline phosphatase (SA-ALP). When capture probes hybridize with miRNAs, duplex-specific nuclease cleaves the formative duplexes. At the moment, the biotin group strips from the electrode surface and SA-ALP is incapacitated to attach onto electrode. Then, ascorbic acids induce the electrochemical chemical chemical redox cycling to produce electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under optimum conditions, the proposed biosensor shows a good linear relationship between the current variation and logarithm of the microRNAs concentration ranging from 0.1 fM to 0.1 pM with a detection limit of 0.086 fM (S/ N=3). Furthermore, the biosensor is successfully applied to detect target miRNA-21 in human serum samples.
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