期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 150, 期 -, 页码 456-464出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2016.11.011
关键词
Microfluidics; Cell adhesion; Shear stress; APTES; PDMS; Surface modification
资金
- Deutscher Akademischer Austauschdienst (DAAD)
- Higher Education Commission (HEC) Pakistan
Microfluidic systems based on polydimethylsiloxane (PDMS) provide a versatile platform to study the mechanoresponse of cells in vitro. Under a shear flow, however, the stability of cells that were grown on physically adsorbed proteins is short lived, which limits long-term cell studies. To address this issue, we used (3-Aminopropyl)triethoxysilane (APTES) as a linker between PDMS and collagen. In micro-channels that were modified with APTES-anchored collagen, fibroblast cells demonstrated higher stability and better proliferation as compared to collagen that was physically adsorbed onto PDMS after oxygen plasma treatment. To assess the stability of the cellular adhesion, cells were forced in a shear flow until detachment. In devices with APTES-anchored collagen, cells showed better adhesion and proliferation at shear stresses between 11.6 and 93 dyn/cm(2) as compared to devices with the adsorbed collagen coating where the first cellular detachment occurred already at a shear stress of 23 dyn/cm(2). The APTES-attached collagen coating also contributed to an improved long-term cellular growth (observed for 48 h) at different shear stress levels (10-300 dyn/cm(2)). Attachment of collagen with the help of APTES thus is a very promising technique not only to modify the glass but also to modify the PDMS surfaces of microfluidic devices for mechanotransduction experiments. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据