期刊
BIO-PROTOCOL
卷 7, 期 6, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2193
关键词
RNA-protein interaction; UV-crosslinking; RNA-binding proteins
类别
资金
- Wellcome Trust-DBT India Alliance Intermediate fellowship [WT500139/Z/09/Z]
- CSIR, India Senior Research Fellowship
RNA-protein interactions play a crucial role in every aspect of RNA metabolism, and also plays a major role in post-transcriptional gene regulation. RNA-binding proteins have been implicated in viral gene expression (Ray and Das, 2002) and microRNA-mediated gene regulation (Poria et al., 2016). Here we have described the protocol which (1) covalently links transiently interacting RNA-protein complexes by UV crosslinking, (2) removes the unprotected RNA by RNase digestion and (3) detects the RNA-protein complexes by SDS-PAGE analysis. This protocol provides a rapid and reliable means to directly assay RNA-protein interactions and their kinetics using purified proteins and also help in identifying novel RNA-protein interactions
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