期刊
ANALYTICAL CHEMISTRY
卷 89, 期 6, 页码 3724-3731出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00044
关键词
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资金
- Ministry of Health Welfare [HI13C1378]
- Global Research Laboratory Program through the National Research Foundation (NRF) - Ministry of Science, ICT & Future Planning, Korea [2014K1A1A2064569]
- Korea Health Promotion Institute [HI13C1378000015] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2014K1A1A2064569] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ratiometric imaging by two-photon microscopy can offer a viable tool for the relative quantification of biological analytes inside tissue with minimal influence from environmental factors that affect fluorescence signal. We demonstrate the ratiometric imaging of formaldehyde at the suborgan level using a two-photon fluorescent probe, which involves pixel-to-pixel ratiometric data transformation. This study reveals for the first time a high level of formaldehyde around the crypts of mouse small intestine, implicating its possible protective role along with the released antimicrobials from the Paneth cells.
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