The Effect of Different Pollination on the Expression of Dangshan Su Pear MicroRNA

The high-throughput sequencing of pear Dangshan Su x Yali (whose fruits lignin and stone cell content are high and quality is poor) and pear Dangshan Su x Wonhwang (whose fruits with low content of lignin and stone cell and the quality are better) found that the expressions of these two miRNAs (pyr-1809 and pyr-novel-miR-144-3p) were significantly different; their corresponding target genes encode two kinds of laccase (Pbr018935.1 and Pbr003857.1). qRT-PCR results showed that these two enzymes are involved in the formation of lignin and stone cells and the existence of these two miRNAs has a negative effect on them. It was concluded that the effect of pollination on the development of stone cells may affect the synthesis of lignin, through the regulation of laccase controlled by miRNAs, and ultimately affect the formation of stone cell and fruit quality.