期刊
ANALYTICA CHIMICA ACTA
卷 959, 期 -, 页码 83-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.12.045
关键词
Rolling circle amplification; Co2+ enhanced ABEI functional flowerlike; gold nanoparticles; WS2 nanosheet; Chemiluminescence resonance energy; transfer; Staphylococcus aureus
资金
- National Natural Science Foundation of China [21375049, 31401576]
- National S&T Support Program of China [2015BAD17B02]
- Key Research and Development Program of Jiangsu Province [BE2016306, BK20140155]
- Synergetic Innovation Center of Food Safety and quality control of Jiangsu Province
A chemiluminescence resonance energy transfer aptasensor was fabricated for the detection of Staphylococcus aureus (S. aureus) with Co2+ enhanced N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (Co2+/ABEI-AuNFs) as donor and WS2 nanosheet as acceptor. In the presence of S. aureus, rolling circle amplification (RCA) can be started. Partially complementary sequence of RCA product functional ABEI-AuNFs (cDNA-ABEI-AuNFs) were then annealed to multiple sites of the RCA product to form duplex complex. This complex is less adsorbed onto the WS2 nanosheet, thus attenuating the quenching of ABEI-AuNFs chemiluminescence by WS2 nanosheet. In the absence of target S. aureus (and hence the absence of RCA and duplex formation), the free cDNA-ABEI-AuNFs is completely adsorbed onto the WS2 nanosheet and chemiluminescence quenching ensues. Under optimal conditions, the logarithmic correlation between the concentration of S. aureus and the CL signal was found to be linear within the range of 50 cfuirnL to 1.5 x 10(5) cfuimL (R-2 = 0.9913). The limits of detection of the developed,method were found to be 15 cfu/mL for S. aureus. The selectivity and the capability of the biosensor in meat samples were also studied. Therefore, this simple and easy operation method can be used to detect S. aureus with high sensitivity and specificity. (C) 2017 Elsevier B.V. All rights reserved.
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