期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 23, 期 -, 页码 196-202出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2017.01.016
关键词
Dunaliella parva; Nitrogen limitation; Lipid production; Proteome; iTRAQ; Differential expression
资金
- Natural Science Foundation of Guangdong Province [2016A030313171, 2016A030312007]
- National Key Research and Development Program-China [2016YFB0601004]
- Pearl River S&T Nova Program of Guangzhou, China [201610010155]
- Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences [y507j21001, y507jb1001]
Nitrogen limitation is the most widely used method for directing metabolic flux to lipid biosynthesis in microalgae. Protein level changes induced by environmental stress could be investigated by proteomics analysis, which was a favourable approach for understanding the induction mechanisms. Although, we previously provided the results of transcriptome sequencing of Dunaliella parva under nitrogen limitation stress, the effects of nitrogen limitation on the microalgal proteome were not reported. The impact of nitrogen limitation on the proteome of green alga D. parva was investigated using iTRAQ quantitative proteomics technique. A total of 227 proteinswere up-regulated, and 159 proteinswere down-regulated under the nitrogen limitation condition. Functional analyses of differentially expressed proteins revealed that nitrogen limitation could induce protein level changes related to photosynthesis, stress response, lipid metabolism, carbohydrate metabolism and nitrogen metabolism. This study, together with our previous transcriptome sequencing of D. parva, therefore provides a foundation for understanding the regulatory mechanism of lipid biosynthesis induced by nitrogen limitation, which could further guide the genetic modification of lipid accumulation in D. parva. (C) 2016 Published by Elsevier B. V.
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