期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 86, 期 -, 页码 354-362出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2016.12.045
关键词
SCI; Electrospinning; ChABC; db-cAMP; PPC
资金
- National Natural Science Foundation of China [30901544, 81572487, 81401867]
- Shandong Provincial Science and Technology Development planning Program of China [2014GSF118019, 2015GSF118074, 2015GSF118061]
- Norwegian Centre for International Cooperation in Education (SIU) [UTF-2014/10047]
- Special foundation for Taishan Scholars [ts20110814, tshw201502056, ts201511093]
- Shandong Provincial Foundation for Distinguished Young Scholars [BS2012YY016]
- Shandong Provincial Natural Science Foundation [ZR2011HM024]
- Fundamental Research Funds of Shandong University [2016JC019]
This study describes the use of poly(propylene carbonate) (PPC) electrospun microfibres impregnated with a combination of dibutyryl cyclic adenosine monophosphate (db-cAMP) and chondroitinase ABC (ChABC) in the treatment of right-side hemisected spinal cord injury (SCI). Release of db-cAMP and/or ChABC from the microfibres was assessed in vitro using high-performance liquid chromatography (HPLC). Drug-impregnated microfibres were implanted into the hemisected thoracic spinal cord of rats, and treatment was evaluated using functional recovery examinations and immunohistochemistry. Our results demonstrated that the microfibres containing db-cAMP and/or ChABC displayed a stable and prolonged release of each agent. Sustained delivery of db-cAMP and/or ChABC was found to promote axonal regenerative sprouting, functional recovery, and reduced glial scar formation when compared to untreated control animals. The combination of both db-cAMP and ChABC was determined to be more effective than using either drug alone in the treatment of SCI. These findings demonstrate the feasibility of using PPC electrospun microfibres for multi-drug combination therapy in SCI. (C) 2016 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据