期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 5, 期 9, 页码 7486-7491出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.7b01203
关键词
Consolidated bioprocessing; Lignin characterization; Clostridium thermocellum; NMR; FTIR
资金
- Renewable BioProducts Institute at Georgia Institute of Technology through the RBI Fellowship
- BioEnergy Science Center a U.S. Department of Energy Bioenergy Research Center - Office of Biological and Environmental Research in the DOE Office of Science
- U.S. Department of Energy [DE-AC05-00OR22725]
During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR The results suggest that C. thermocellum reduces the beta-O-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.
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