期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 6, 期 1, 页码 364-373出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.7b02610
关键词
Green synthesis of AuNPs; Nucleic acids; Guanine; Nucleic acid oxidation; 8-OHdG; RNA in Leptothrix sheaths
资金
- JST-CREST
- JSPS [16K07714]
- Grants-in-Aid for Scientific Research [16K07714] Funding Source: KAKEN
Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2'-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2'-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.
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