期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 119, 期 -, 页码 34-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2016.12.005
关键词
Hydrophobic charge-induction; chromatography; Competitive adsorption; Immunoglobulin G; Bovine serum albumin; Adsorption isotherms; Protein separation
资金
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation [512100-X91603]
Hydrophobic charge-induction chromatography (HCIC) with 2-mercapto-1-methyl-imidazole (MMI) ligands shows promising results in antibody purification. In this study, competitive adsorption processes between bovine immunoglobulin G (IgG) and bovine serum albumin (BSA) were investigated with MMI resins. Adsorption isotherms of IgG, BSA and IgG/BSA mixtures at various mass ratios were measured, and the Langmuir-Freundlich model was used to fit the adsorption experimental data. The results indicated that IgG could be preferentially adsorbed by MMI resins, and MMI-B-4FF-100 resin with the pore size of 43.2 nm and the ligand density of 101 mu mol/g gel showed the best adsorption selectivity of IgG over BSA. In addition, binding behaviors of IgG and BSA in column were studied with MMI-B-4FF-100 at various pH values, and the optimized separation conditions were obtained as loading at pH 7.0 and elution at pH 4.0. Furthermore, IgG was effectively separated from IgG/BSA protein mixtures with MMI-B-4FF-100. The purities of IgG monomer reached above 95%, and the recovery kept about 90%. The results indicated that MMI-B-4FF-100 with high ligand density and moderate pore size would be a promising HCIC resin for IgG purification. (C) 2016 Elsevier B.V. All rights reserved.
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