期刊
AQUACULTURE
卷 470, 期 -, 页码 68-73出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2016.12.028
关键词
Vibrio sp V33; Vibrio splendidus Vs; Bacterial antagonist; Siderophore production; Protective effect
资金
- National Natural Science Foundation of China [41676141, 31522059, 41576139]
- Program of Science and Technology of Ningbo City of China [2015C50057]
- Public Welfare Project of Zhejiang
- K.C. Wong Magna Fund in Ningbo University
Recently, the use of probiotics as an alternative strategy to control bacterial diseases in aquaculture has garnered much attention due to the merits of environmental friendliness. In this study, a bacterium identified as Vibrio sp. V33 was isolated from healthy sepia and was determined to have strong antagonistic activity towards the pathogenic isolate Vibrio splendidus Vs using the disk diffusion method. The antagonistic substance was secreted into the supernatant and was thermo-stable. The antagonistic activity of Vibrio sp. V33 may be attributed to its higher ability to compete for iron than that of V. splendidus Vs, as deduced from siderophore quantification using the CAS method. Number of colony counts from seawater and Apostichopus japonicus infection experiments showed that Vibrio sp. V33 significantly decreased the quantity of V. splendidus Vs in natural seawater and promoted the survival rate of A. japonicus infected by V. splendidus, with a relative percentage survival of 43% under the current conditions. Our findings suggest that V33 could be potentially used as potential bacterial antagonist for controlling V. splendidus infections in sea cucumber aquaculture. Statement of relevance: It might protect A. japonicus from V. splendidus infection. (C) 2016 Elsevier B.V. All rights reserved.
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