期刊
TOXINS
卷 9, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/toxins9030077
关键词
type IV toxin; antitoxin; prophage; Escherichia coli
资金
- National Science Foundation of China [31270214, 31290233, 31400162, 41406189]
- Chinese Academy of Sciences [XDA11030402]
- 1000-Youth Elite Program (the Recruitment Program of Global Experts in China)
Toxin/antitoxin (TA) systems are widespread in prokaryotic chromosomes and in mobile genetic elements including plasmids and prophages. The first characterized Type IV TA system CbtA/CbeA was found in cryptic prophage CP4-44 in Escherichia coli K-12. Two homologous TA loci of CbtA/CbeA also reside in cryptic prophages of E. coli K-12, YkfI/YafW in CP4-6 and YpjF/YfjZ in CP4-57. In this study, we demonstrated that YkfI and YpjF inhibited cell growth and led to the formation of lemon-shaped cells. Prolonged overproduction of YkfI led to the formation of gourd-shaped cells and immediate cell lysis. YafW and YfjZ can neutralize the toxicity of YkfI or YpjF. Furthermore, we found that YkfI and YpjF interacted with cell division protein FtsZ in E. coli, but ectopic expression in Pseudomonas and Shewanella did not cause the formation of lemon-shaped cells. Moreover, deletion of all of the three toxin genes together decreased resistance to oxidative stress and deletion of the antitoxin genes increased early biofilm formation. Collectively, these results demonstrated that the homologous Type IV TA systems in E. coli may target cell division protein FtsZ in E. coli and may have different physiological functions in E. coli.
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