期刊
TOXINS
卷 9, 期 7, 页码 -出版社
MDPI AG
DOI: 10.3390/toxins9070232
关键词
histone methylation; sclerotia; stress response; fungal virulence
资金
- National Natural Science Foundation of China [31172297, 2013CB127802]
- Molecular Cell and Systems Biology, Life Science College of FAFU
Lysine methyltransferases transfer methyl groups in specific lysine sites, which regulates a variety of important biological processes in eukaryotes. In this study, we characterized a novel homolog of the yeast methyltransferase DOT1 in A. flavus, and observed the roles of dot1 in A. flavus. Deletion of dot1 showed a significant decrease in conidiation, but an increase in sclerotia formation. A change in viability to multiple stresses was also found in the Delta dot1 mutant. Additionally, aflatoxin (AF) production was found severely impaired in the Delta dot1 mutant. Further analysis by qRT-PCR revealed that the transcription of AF structural genes and their regulator gene aflS were prominently suppressed in the Delta dot1 mutant. Furthermore, our data revealed that Dot1 is important for colonizing maize seeds in A. flavus. Our research indicates that Dot1 is involved in fungal development, aflatoxin biosynthesis and fungal virulence in A. flavus, which might provide a potential target for controlling A. flavus with new strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据