期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 6, 期 10, 页码 1840-1851出版社
OXFORD UNIV PRESS
DOI: 10.1002/sctm.17-0028
关键词
Multiple sclerosis; Neuroinflammation; Central nervous system; Mesenchymal stromal cell; Pericyte; Perivascular niche; Blood vessels
资金
- Stockholm County Council
- Swedish Research Council
- Max and Edith Follins Foundation
- Karolinska Institutet
Vascular changes, including blood brain barrier destabilization, are common pathological features in multiple sclerosis (MS) lesions. Blood vessels within adult organs are reported to harbor mesenchymal stromal cells (MSCs) with phenotypical and functional characteristics similar to pericytes. We performed an immunohistochemical study of MSCs/pericytes in brain tissue from MS and healthy persons. Post-mortem brain tissue from patients with early progressive MS (EPMS), late stage progressive MS (LPMS), and healthy persons were analyzed for the MSC and pericyte markers CD146, platelet-derived growth factor receptor beta (PDGFR beta), CD73, CD271, alpha-smooth muscle actin, and Ki67. The MS samples included active, chronic active, chronic inactive lesions, and normal-appearing white matter. MSC and pericyte marker localization were detected in association with blood vessels, including subendothelial CD146(+)PDGFR(+)Ki67(+) cells and CD73(+)CD271(+)PDGFR beta(+)Ki67(-) cells within the adventitia and perivascular areas. Both immunostained cell subpopulations were termed mesenchymal perivascular cells (MPCs). Quantitative analyses of immunostainings showed active lesions containing increased regions of CD146(+)PDGFR beta(+)Ki67(+) and CD73(+)CD271(+)PDGFR beta(+)Ki67(-) MPC subpopulations compared to inactive lesions. Chronic lesions presented with decreased levels of CD146(+)PDGFR beta(+)Ki67(+) MPC cells compared to control tissue. Furthermore, LPMS lesions displayed increased numbers of blood vessels harboring greatly enlarged CD73(+)CD271(+) adventitial and perivascular areas compared to control and EPMS tissue. In conclusion, we demonstrate the presence of MPC subgroups in control human brain vasculature, and their phenotypic changes in MS brain, which correlated with inflammation, demyelination and MS disease duration. Our findings demonstrate that brain-derived MPCs respond to pathologic mechanisms involved in MS disease progression and suggest that vessel-targeted therapeutics may benefit patients with progressive MS.
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