期刊
CHEMICAL COMMUNICATIONS
卷 53, 期 6, 页码 1124-1127出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc08726d
关键词
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资金
- National Natural Science Foundation of China [21335005, 21472120, 21622507]
- rogram for Changjiang Scholars and Innovative Research Team in University [IRT_ 15R43]
- Natural Science Basic Research Plan of Shaanxi Province [2015KJXX-22]
- Fundamental Research Funds for the Central Universities [GK201501003, GK201603039]
Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.
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