期刊
CELL RESEARCH
卷 27, 期 4, 页码 505-525出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2017.42
关键词
T-cell receptor; lipid; conformational dynamics; atomic force microscopy; nuclear magnetic resonance; live-cell imaging
类别
资金
- Chinese Academy of Sciences (Strategic Priority Research Program) [XDB08020100]
- National Natural Science Foundation of China [31370860, 31425009, 31530022, 31621003, 31470734, 21373200]
- Ministry of Science and Technoloy of China [2014CB541903, 2011CB933600]
- China Postdoctoral Science Foundation [2015M580357]
T-cell receptor-CD3 complex (TCR) is a versatile signaling machine that can initiate antigen-specific immune responses based on various biochemical changes of CD3 cytoplasmic domains, but the underlying structural basis remains elusive. Here we developed biophysical approaches to study the conformational dynamics of CD3 epsilon cytoplasmic domain (CD3 epsilon(CD)). At the single-molecule level, we found that CD3 epsilon(CD) could have multiple conformational states with different openness of three functional motifs, i.e., ITAM, BRS and PRS. These conformations were generated because different regions of CD3 epsilon(CD) had heterogeneous lipid-binding properties and therefore had heterogeneous dynamics. Live-cell imaging experiments demonstrated that different antigen stimulations could stabilize CD3 epsilon(CD) at different conformations. Lipid-dependent conformational dynamics thus provide structural basis for the versatile signaling property of TCR.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据