期刊
ANNALS OF MICROBIOLOGY
卷 67, 期 4, 页码 333-342出版社
BMC
DOI: 10.1007/s13213-017-1264-4
关键词
Coconut fiber; Pectinase; Pectin lyase; Solid state fermentation; Optimization
资金
- University Grants Commission, India as Maulana Azad National Senior Research Fellow under Maulana Azad National Fellowship
Pectin-degrading enzymes (pectinase and pectin lyase) were produced in solid state fermentation by Bacillus subtilis SAV-21 isolated from fruit and vegetable market waste soil of Yamuna Nagar, Haryana, India, and identified by 16S rDNA sequencing. Under optimized conditions, maximum production of pectinase (3315 U/gds) and pectin lyase (10.5 U/gds) was recorded in the presence of a combination of orange peel and coconut fiber (4: 1), with a moisture content of 60% at 35 degrees C and pH 4.0 after 4 days and 8 days of incubation, respectively. Pectinase yield was enhanced upon supplementation with galactose and yeast extract, whereas pectin lyase production was unaffected by adding carbon and nitrogen source to the basal medium. Thus, B. subtilis SAV-21 can be exploited for cost-effective production of pectinase and pectin lyase using agro-residues.
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