期刊
BIOCONJUGATE CHEMISTRY
卷 28, 期 4, 页码 897-902出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.7b00024
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- Nanyang Technological University
- Singapore Ministry of Education [MOE2013-T2-1-060, RG2/15, RG11/13, RG35/15]
Herein, we report use of 2-azidoacrylates to perform site-specific dual functionalization of the cysteine residue of peptides and bovine serum albumin (BSA), a native protein containing one free cysteine residue. The sulfhydryl group of the cysteine residue could be conjugated with 2-azidoacrylates bearing various functionalities, such as fluorescent dyes under physiological aqueous buffer conditions, to afford peptide and protein conjugates anchoring an azide moiety. Successive azide-alkyne cycloaddition enables installation of the second functionality,thus affording dual-functionalized peptide- and protein-based materials.
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