期刊
CHEMICAL ENGINEERING JOURNAL
卷 313, 期 -, 页码 1215-1221出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2016.11.021
关键词
TCE; Perchlorate; Simultaneous reductive dechlorination; Dehalococcoides; Oxygen
资金
- National Natural Science Foundation of China [21107091, 21377109, 21577123]
- Natural Science Funds for Distinguished Young Scholar of Zhejiang Province [LR17B070001]
- Science and Technology Department of Zhejiang Province [2015033016]
We enriched a trichloroethene (TCE)-dechlorinating consortium from a perchlorate-reducing bacterial culture (PRB) with a very low initial abundance of Dehalococcoides by feeding the culture lactate and TCE; named the novel culture perchlorate-reducing dechlorinating bacteria (PRTB). Short batch tests showed that the PRTB consortium reduced up to 0.45 mmol/L of TCE to non-toxic ethene in eight days after two additions of TCE and simultaneously reduced up to 0.15 mmol/L of CLO-4 to CI-. TCE reduction by PRTB was sensitive to oxygen: 0.2 mg/L of oxygen delayed reduction of TCE to ethene from 5 to 15 days, while >= 0.5 mg/L of oxygen significantly inhibited TCE reduction, causing vinyl chloride accumulation. After the consortium was enriched twice with TCE, the Dhc gene (reflecting the abundance of Dehalococcoides) increased from 1.14 Chi 10(4) to 3.25 Chi 10(8) copies/mL. The original PRB culture was dominated by Proteobacteria (78.4%), but the enriched PRTB culture was dominated by Chloroflexi (57.7%). The relative abundance of Dehalococcoides increased from 0.02% to 57.4%. Other presumptive dechlorinators Desulfuromonas and Geobacter were enriched as well. Possible acetogens (Spirochaetes) and putative fermenters (Bacteroidetes and Desulfovibrio) were also present in the culture, providing Dehalococcoides with acetate as the carbon source, hydrogen gas as the electron donor, and Vitamin B-12 as a growth factor. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据