期刊
BIOELECTROCHEMISTRY
卷 114, 期 -, 页码 48-53出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2016.12.006
关键词
Conductive hydrogel; Electrochemical immunosensor; Neuron-specific enolase; Tumor biomarker; 1,3,5-Benzenetricarboxylic acid; Iron ion
资金
- National Natural Science Foundation of China [21673143, 21273153]
- Beijing Natural Science Foundation of China [2132008]
- Project of the Construction of Scientific Research Base by the Beijing Municipal Education Commission
In this work, a new conductive hydrogel was prepared by a simple cross-linking coordination method using 1,3,5-benzenetricarboxylic acid as the ligand and Fe3+ as the metal ion. The hydrogel film was formed on a glassy carbon electrode (GCE) by a drop coating method, which can dramatically facilitate the transport of electrons. A sensitive label-free electrochemical immunosensor was fabricated following electrodeposition of gold nanoparticles (AuNPs) on a hydrogel film and immobilization of an antibody. Neuron-specific enolase (NSE), a lung cancer biomarker, was used as the model analyte to be detected. The proposed immunosensor exhibited a wide linear detection range of 1 pg mL(-1) to 200 ng mL(-1) and a limit of detection of 026 pg mL(-1) (the ratio of signal to noise (S/N) = 3). Moreover, the detection of NSE in human serum samples showed satisfactory accuracy compared with the data determined by enzyme-linked immunosorbent assay (ELISA), indicating good analytical performance of the immunoassay. (C) 2016 Elsevier B.V. All rights reserved.
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