期刊
BIOMATERIALS
卷 122, 期 -, 页码 130-140出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2017.01.020
关键词
Specific phototherapy; NIR bioimaging; Enzyme-activated fluorescence; Multiple functional probe
资金
- National Creative Research Initiative Program [2012R1A3A2048814]
- Korea Mouse Phenotyping Center [2016M3A9D5A01952416]
- Brain Korea 21 PLUS Project for Medical Science, Yonsei University
- Bio and Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science, ICT and Future Planning [NRF-2013M3A9D5072551]
- National Natural Science Foundation of China [21376117]
- Jiangsu Natural Science Funds for Distinguished Young Scholars [BK20140043]
- Natural Science Foundation of Jiangsu Higher Education Institutions of China [14KJA150005]
The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation. (C) 2017 Elsevier Ltd. All rights reserved.
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