Polyhydroxyalkanoate production from crude glycerol by newly isolated Pandoraea sp.

A new bacterial strain was isolated from Atlantic rainforest in Brazil for polyhydrox-yalkanoate (PHA) production utilizing crude glycerol from biodiesel industry (CG) and it was identified as Pandoraea sp. MA03. Shake flask experiments were performed at 10-50 g L-1 carbon source and showed the best values of poly(3-hydroxybutyrate) (P3HB) production from CG cultivations compared to pure glycerol, with a polymer accumulation ranging from 49.0% to 63.6% cell dry weight (CDW). The results obtained from this study showed a positive effect of contaminant NaCl on P3HB synthesis up to 30 g L-1 CG. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P (3HB-co-3HV)] production was obtained from CG plus propionic acid with up to 25.9 mol% 3HV. Since it is interesting the utilization of CG for obtaining added-value products along with biodiesel, this study reported a novel and promising PHA-producing bacterial strain as an additional effort to enhance the viability of a sustainable industry based on biofuels and biopolymers. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.