期刊
CELLULOSE
卷 24, 期 4, 页码 1901-1914出版社
SPRINGER
DOI: 10.1007/s10570-017-1231-z
关键词
Softwood; Lignin modification; Lignocellulose; Pyrolysis-molecular beam mass spectroscopy; Solid-state NMR
资金
- U. S. Department of Agriculture (USDA) [87-FSTY-9-0278, NRICGP 92-37301-7593, 95-37103-2061]
- U. S. Department of Energy (DOE) [DE-FG02-03ER15442]
- National Science Foundation [IBN-9118386, DBI-0922391]
- USDA National Institute of Food and Agriculture (NIFA) National Needs Fellowship (NNF) [2012-38420-30203]
- DOE Office of Science, Office of Workforce Development for Teachers and Scientists, Office of Science Graduate Student Research (SCGSR) program
- DOE [DE-AC05-06OR23100]
- Office of Biological and Environmental Research in the DOE Office of Science
Softwood is an abundant resource; how-ever, currently its utilization for bioconversion to obtain platform sugars is limited. Pinus taeda trees which were genetically modified to either produce S lignin or to decrease lignin content were character-ized with a suite of analytic techniques. Syringyl lignin was visualized in the secondary xylem of one genetic line with Maule staining. Solid-state nuclear magnetic resonance identified the S lignin units were coupled into the lignin through beta-O-4 linkages, and thioacidolysis measured approximately 13% S lignin content in the same sample. Reductions of the lignin of as much as 33% were observed in the transgenics. To better understand how these modifications affect bioconversion, their amenability to hot water and dilute acid pretreatments and enzymatic hydrolysis was evaluated. Lignin reductions resulted in 1.9-3.2-fold increases in glucose release compared to the control. However, no apparent benefit was observed by S lignin incorporation at the concentrations reported in this study. These results highlight the potential for softwood cell wall properties to be improved for bioenergy/biochemical applications.
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