期刊
ANALYST
卷 142, 期 6, 页码 945-950出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6an02441f
关键词
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资金
- Russian Science Foundation [14-24-00061]
- Russian Science Foundation [17-24-00003] Funding Source: Russian Science Foundation
Molecular beacons (MBs) are valuable tools in molecular biology, clinical diagnostics and analytical chemistry. Here we describe a novel approach for the design of MBs with nucleotide or non-nucleotide linkers between the stem and loop regions. Such modified MBs have significantly improved specificity and performance for single nucleotide polymorphism (SNP) detection. These advantages are especially distinct, when compared to the classic MBs, in the case of possible interactions between the stem and loop regions. We demonstrated the applicability of such modified MBs for the discrimination of common Factor V, NOS3 and ADRB2 SNPs in model plasmids and in clinical samples. The developed approach could be applicable not only to fluorescently labeled MBs, but also to other biosensors based on nucleic acids with stem-loop structures.
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