期刊
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
卷 12, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13018-017-0536-9
关键词
Ischemia reperfusion injury; Neuroprotection; Oxidative stress; Oxygen glucose deprivation; Simvastatin; Spinal cord injury
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF)-Ministry of Education, Republic of Korea. [800-2014-0159]
Background: Ischemia and the following reperfusion damage are critical mechanisms of spinal cord injury. Statins have been reported to decrease ischemia-reperfusion injury in many organs including the spinal cord. Anti-oxidative effect is one of the main protective mechanisms of statin against neuronal death and cytotoxicity. We hypothesized that statins' anti-oxidative property would yield neuroprotective effects on spinal cord ischemia-reperfusion injury Methods: Primary cultured spinal cord motor neurons were isolated from Sprague-Dawley rat fetuses. Ischemiareperfusion injury model was induced by 60 min of oxygen and glucose deprivation (OGD) and 24 h of reoxygenation. Healthy and OGD cells were treated with simvastatin at concentrations of 0.1, 1, and 10 mu M for 24 h. Cell viability was assessed using water-soluble tetrazolium salt (WST)-8, cytotoxicity with LDH, and production of free radicals with DCFDA (2',7'-dichlorofluorescein diacetate). Results: OGD reduced neuronal viability compared to normoxic control by 35.3%; however, 0.1-10 mu M of simvastatin treatment following OGD improved cell survival. OGD increased LDH release up to 214%; however, simvastatin treatment attenuated its cytotoxicity at concentrations of 0.1- 10 mu M (p < 0.001 and p = 0.001). Simvastatin also reduced deteriorated morphological changes of motor neurons following OGD. Oxidative stress was reduced by simvastatin (0.1- 10 mu M) compared to untreated cells exposed to OGD (p < 0.001). Conclusions: Simvastatin effectively reduced spinal cord neuronal death and cytotoxicity against ischemia-reperfusion injury, probably via modification of oxidative stress.
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