期刊
BIORESOURCE TECHNOLOGY
卷 228, 期 -, 页码 250-256出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.12.111
关键词
Methane; Carbon dioxide; Co-culture; Biogas; Natural gas; Methanotroph; Cyanobacteria
资金
- Linus Pauling Distinguished Postdoctoral Fellowship Program, Laboratory Directed Research and Development Program of Pacific Northwest National Laboratory
- DOE OBER
- [49356]
A new co-cultivation technology is presented that converts greenhouse gasses, CH4 and CO2, into microbial biomass. The methanotrophic bacterium, Methylomicrobium alcaliphilum 20z, was coupled to a cyanobacterium, Synechococcus PCC 7002 via oxygenic photosynthesis. The system exhibited robust growth on diverse gas mixtures ranging from biogas to those representative of a natural gas feedstock. A continuous processes was developed on a synthetic natural gas feed that achieved steady-state by imposing coupled light and O-2 limitations on the cyanobacterium and methanotroph, respectively. Continuous co-cultivation resulted in an O-2 depleted reactor and does not require CH4/O-2 mixtures to be fed into the system, thereby enhancing process safety considerations over traditional methanotroph mono-culture platforms. This co-culture technology is scalable with respect to its ability to utilize different gas streams and its biological components constructed from model bacteria that can be metabolically customized to produce a range of biofuels and bioproducts. (C) 2017 Battelle Memorial Institute. Published by Elsevier Ltd.
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