期刊
JOURNAL OF INTEGRATIVE AGRICULTURE
卷 16, 期 9, 页码 2019-2028出版社
ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(16)61582-4
关键词
porcine; microRNA-34c; FoxO3a; granulosa cell function
资金
- National Natural Science Foundation of China [31201771]
- earmarked fund for the China Agriculture Research System [CARS-36]
Granulosa cells (GCs) are somatic cells of ovary, the behaviors of GCs are important for ovarian function. MicroRNAs (miRNAs) are a class of endogenous 18-24 nucleotide (nt) non-coding RNAs, some of which have been shown to be important regulators of GCs function. miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types. However, the roles of miR-34c in GCs function remain unknown. In this study, we used Annexin V-FITC and EdU assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs. Dual-luciferase reporter assays, quantitative real-time PCR (qRT-PCR) and Western blotting identified Forkhead box O3a (FoxO3a) as a direct target gene of miR-34c. The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs. In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a.
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