期刊
JOURNAL OF INTEGRATIVE AGRICULTURE
卷 16, 期 8, 页码 1874-1878出版社
ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(16)61531-9
关键词
Staphylococcus aureus; penicillin-resistant; beta-lactamase; biofilm; ica
资金
- Natural Science Foundation of Gansu Province, China [145RJYA311]
- Central Public-Interest Scientific Institution Basal Research Fund, China [1610322015007]
- Key Technologies R&D Program of China [2012BAD12B03]
Bovine mastitis caused by Staphylococcus aureus is difficult to treat because of increasing resistance against antibiotics, especially penicillin. beta-Lactamase and biofilm are responsible for penicillin resistance of S. aureus. The aim of this study was to investigate the beta-lactamase activity and biofilm formation capacity of 37 penicillin-resistant S. aureus strains (35 were blaZ positive and 2 were blaZ negative) from bovine mastitis in Gansu Province, China, as well as to measure the intercellular adhesion genes icaA and icaD of these strains. beta-Lactamase Test Kit was used to determine the beta-lactamase activity, biofilm formation was tested by semi-quantitative adherence assay method. Moreover, the presence of icaA and icaD were measured by PCR. A total of 32 penicillin-resistant S. aureus strains, including the two blaZ-negative strains, were identified as beta-lactamase producers. All tested S. aureus isolates produced biofilm in the microtiter plate assay. Meanwhile, all these strains were PCR-positive for the ica locus, icaA and icaD. The study indicated high prevalence of beta-lactamase activity, biofilm-forming capacity, and the ica genes among the penicillin-resistant S. aureus isolates, and implied that S. aureus resistant to penicillin was attributed to multiple mechanisms.
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