期刊
JOURNAL OF GINSENG RESEARCH
卷 41, 期 4, 页码 608-614出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2017.05.006
关键词
ginsenoside Rg1; myoblast differentiation; myogenic conversion; myotube hypertrophy; promyogenic signaling
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2011-0030074, 2016R1A2B4014868]
- Korea Institute of Science and Technology [2Z04950]
- National Research Foundation of Korea [2016R1A2B4014868] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
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