期刊
JOURNAL OF GINSENG RESEARCH
卷 42, 期 4, 页码 412-418出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2017.04.007
关键词
beta-glucosidase; bioconversion; compound K; ginsenoside Rg3(S); Lactococcus lactis
资金
- Intelligent Synthetic Biology Center of Global Frontier Project - Korean Ministry of Science, ICT, and Future Planning [2013M3A6A8073553]
- National Research Foundation of Korea (NRF) grant - Korean government (MEST) [2015R1A2A2A01007156]
Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors beta-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific beta-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
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