期刊
BMC MICROBIOLOGY
卷 17, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12866-017-0986-6
关键词
Genomic Island; Pathogenicity Island; B. cenocepacia AU1054; Virulence factor
类别
资金
- National Natural Science Foundation of China [31470068]
- Sichuan Youth Science and Technology Foundation of China [2014JQ0051]
- Fundamental Research Funds for the Central Universities of China [ZYGX2015J144]
- Strategic Research Theme Fund
- The University of Hong Kong
- Croucher Senior Medical Research Fellowship
Background: Genomic islands (GIs) are genomic regions that reveal evidence of horizontal DNA transfer. They can code for many functions and may augment a bacterium's adaptation to its host or environment. GIs have been identified in strain J2315 of Burkholderia cenocepacia, whereas in strain AU 1054 there has been no published works on such regions according to our text mining and keyword search in Medline. Results: In this study, we identified 21 GIs in AU 1054 by combining two computational tools. Feature analyses suggested that the predictions are highly reliable and hence illustrated the advantage of joint predictions by two independent methods. Based on putative virulence factors, four GIs were further identified as pathogenicity islands (PAIs). Through experiments of gene deletion mutants in live bacteria, two putative PAIs were confirmed, and the virulence factors involved were identified as lipA and copR. The importance of the genes lipA (from PAI 1) and copR (from PAI 2) for bacterial invasion and replication indicates that they are required for the invasive properties of B. cenocepacia and may function as virulence determinants for bacterial pathogenesis and host infection. Conclusions: This approach of in silico prediction of GIs and subsequent identification of potential virulence factors in the putative island regions with final validation using wet experiments could be used as an effective strategy to rapidly discover novel virulence factors in other bacterial species and strains.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据