期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 25, 期 6, 页码 1770-1777出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2017.01.033
关键词
Cancer; Metal based anticancer agents; Palladium(II) complex; Cell cycle specific; Apoptosis; Cytotoxicity; Cell cycle arrest
资金
- internal TUBITAK
- Istanbul University Research Fund [36696]
Objectives: Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. Materials and methods: Cells were arrested at Gl, Gl/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. Results: While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. Conclusions: Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents. (C) 2017 Elsevier Ltd. All rights reserved.
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